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12. | | ANDRADE, M. A. RIBEIRO, B. M. CASTRO, M. E. B. Construção e caracterização de uma linhagem celular estável, ufl-ag-pac, contendo o gene inibidor de apoptose iap-3 de orgyia pseudotsugata nucleopolyhedrovirus (OpMNPV). ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 35 Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | SOARES, E. F.; RIBEIRO, B. M.; CASTRO, M. E. B. Construção de um vírus recombinante do mutante de Anticarsia gemmatalis nucleopolyhedrovirus, vApAg contendo a proteína fluorescente GFP. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 57. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | CARPES, M. P.; RIBEIRO, B. M.; CASTRO, M. E. B. Localização, clonagem e sequenciamento do gene inibidor de apoptose iap-3 de Anticarsia gemmatalis Nucleopolyhedrovirus (AgMNPV). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6., 2001, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2001. p. 41. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
01/07/2004 |
Data da última atualização: |
26/07/2017 |
Autoria: |
PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M. |
Título: |
Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Biological Control, v. 27, n. 3, p. 336-344, July 2003. |
Idioma: |
Inglês |
Conteúdo: |
Some baculovirus have been genetically modified for the inactivation of their ecdysteroid
glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type
AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A.
gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. |
Palavras-Chave: |
Ecdysteroid glucosyltransferase; Velvetbean caterpillar. |
Thesagro: |
Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02224naa a2200241 a 4500 001 1467192 005 2017-07-26 008 2003 bl --- 0-- u #d 100 1 $aPINEDO, F. J. R. 245 $aInactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. 260 $c2003 520 $aSome baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. 650 $aAnticarsia Gemmatalis 650 $aBaculovirus 650 $aControle Biológico 650 $aEngenharia Genética 653 $aEcdysteroid glucosyltransferase 653 $aVelvetbean caterpillar 700 1 $aMOSCARDI, F. 700 1 $aLUQUE, T. 700 1 $aOLSZEWSKI, J. A. 700 1 $aRIBEIRO, B. M. 773 $tBiological Control$gv. 27, n. 3, p. 336-344, July 2003.
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